Mechanisms of action of natural insecticides and acaricides; expression of octopamine and tyramine receptors in mammalian cell lines


Octopamine and tyramine are essential biogenic amines that have been implicated in numerous physiological systems in arthropod species, such as reproduction, the nervous system, and  learning-and-memory, to name a few. We have successfully created two stably-transfected Chinese Hamster Ovary cell lines (CHO) with a functional octopamine receptor from Periplaneta americana, the American cockroach, and a functional tyramine receptor from Rhipicephalus microplus, the southern cattle tick. Our hope is to characterize plant-derived compounds that may function as agonists, antagonists, or modulators of these two arthropod receptors. This research may lead to the identification of plant-derived compounds that possess activity at these two receptors and may act as insecticidal compounds with a novel mode of action that has yet to be exploited in the field of insect toxicology.Photograph of Chinese Hamster ovary cells


Some relevant publications:

Gross, Aaron D., Michael J. Kimber, Tim A. Day, Paula Ribeiro, and Joel R. Coats. 2013. Quantitative structure-activity relationships (QSARs) of monoterpenoids at an expressed American cockroach octopamine receptor, Chapter 7 in Pest Management with Natural Products, J.J. Beck, J.R. Coats, S.O. Duke and M.E. Koivunen, Editors, American Chemical Society, Washington, D.C. 247 pp. DOI: 10.102/bk-2013-1141.ch007.

Tsao, R., S. Lee, P.J. Rice, C. Jensen, and J.R. Coats. 1995. Monoterpenoids and their synthetic derivatives as leads for new insect-control agents.  Chapter 28, in Synthesis and Chemistry of Agrochemicals - IV, D.R. Baker, J.G. Fenyes, and G.S. Basarab, eds., pp. 312-324.  American Chemical Society, Washington, D.C.