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Pesticide Toxicology Laboratory

ISU Entomology / Pesticide Toxicology Laboratory / Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans.

Bacillus thuringiensis (Bt) toxin susceptibility and isolation of resistance mutants in the nematode Caenorhabditis elegans.

Authors: Marroquin, L.D, Elyassnia, D., Griffitts, J.S., Feitelson, J.S., Aroian, R.V.
Journal, Volume, Year: Genetics, volume 155:1693-1699, 2000
Summary

Studied Cry5B and Cry6A Bt toxin action against Caenorhabditis elegans. Fed nematodes diet with 12.5 microgram/mL Cry5B from Bt lysates or E. coli. Cry5B was found to be toxic to wild-type C. elegans with an LC50 of 12.6 microgram/mL. Brood size was reduced in all strains of C. elegans (even resistant mutants). Mutants were also isolated that were resistant to Cry5B. These mutants had very low mortality in two strains and no mortality in three strains, at 420 microgram/mL. Resistant strains were susceptible to Cry6A, however.

Comments

Showed potential for use of a type of Bt against nematodes. Cry5B has sequence similarity to Cry1Ab. Also showed that the mechanism of action for Cry5B and Cry6A is probably different.