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Pesticide Toxicology Laboratory

ISU Entomology / Pesticide Toxicology Laboratory / Quantitative analysis of CryIA(b) expression in Bt maize plants, tissues, and silage and stability of expression over successive generations

Quantitative analysis of CryIA(b) expression in Bt maize plants, tissues, and silage and stability of expression over successive generations

Authors: Fearing, P.L., Brown, D., Vlachos, D., Meghji, M., Privalle, L.
Journal, Volume, Year: Molecular Breeding, volume 3:169-176, 1997
Summary

Study quantifying expression of Bt Cry 1A(b) in plant tissues, pollen, leaves, and silage using ELISA. Found that the highest level of expression was at antithesis. Only leaves and pollen had >10 ng toxin/g material.

Comments

Valuable information on ELISA analysis and concentration of toxin in plant parts.