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Pesticide Toxicology Laboratory

ISU Entomology / Pesticide Toxicology Laboratory / Monitoring the insecticidal toxins from Bacillus thuringiensis in soil with flow cytometry

Monitoring the insecticidal toxins from Bacillus thuringiensis in soil with flow cytometry

Authors: Tapp, H., Stotzky, G.
Journal, Volume, Year: Canadian Journal of Microbiology, volume 43:1074-1078, 1997
Summary

This paper gives a method for determining the presence of the toxin from Bt var. tenebrionis and Bt var. kurstaki on clay and silt-sized particles from soil. Toxin was bound to soil and a buffer was used to block non-specific antibody binding. Antibody with a fluorescent marker was added to the suspension. Flow cytometry was used for detection.

Comments

They did not use real soil. There was considerable cross-reactivity potential, especially in environmental matrices. Authors say that it is more sensitive and rapid than ELISA.