Quantification in soil of Bacillus thuringiensis var. kurstaki delta-endotoxin from transgenic plants
Authors: Palm, C.J., Donegan, K., Harris, D., Seidler, R.J.
Journal, Volume, Year: Molecular Ecology, volume 3:145-151, 1994
This paper studies the fate of Bt var kurstaki from transgenic cotton. Authors used a high pH, high salt buffer extraction and ELISA to quantify the toxin in natural soil. Toxin levels in soil decreased sharply for the first 10 days but then held constant for the next 20 days (30-day test). Authors state that protein from plant decays faster than purified.
One of the few studies to use natural soil and a transgenic source of toxin. Showed possibility of a persistent percentage of toxin in the soil. The extraction used was very inefficient with 60% recovery being the best and 27% the worst. Statement inidcating that purified protein is more stable in soil than plant-produced is misleading. In the data shown, the starting amounts of the plant-produced protein were much higher than those of the purified protein, which definitely affects rate of decay or dissipation.