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gene cloning

Cloning and expression of a venom protein from the endoparasitoid, Pimpla hypochondriaca, which has haemocyte anti-aggregation activity in vitro

Venom from the endoparasitoid, Pimpla hypochondriaca contains a mixture of proteins. One of these was previously biochemically isolated and shown to have haemocyte anti-aggregation activity against host haemocytes in vitro. This protein shares significant homology to a second venom protein (VPB) from this parasitoid. The gene for VPB was amplified from a P. hypochondriaca venom gland cDNA library by PCR, cloned and expressed in E. coli. The presence of a fusion tag allowed purification. The purified immunosuppressive protein was found to have anti-haemocyte activity, in vitro, against haemocytes from two lepidopteran pests. This venom protein may have the potential to improve efficacy of biocontrol agents.

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