38th Annual Meeting of the Society for Invertebrate Pathology

Study of life cycles and transmission of parasites included experiments cross-infections between mosquito larvae and co-inhabiting hydrobionts. Spores of Parathelohania, Amblyospora and Trichoctosporea species from Anopheles and Aedes mosquitoes were used for per oral infections of nauplial, copepodite stages and adult crustaceans (16 species of Cyclopoida, 3 species of Calanoida) and insects (larvae of a dragon-fly and a may-fly). In the same water-bodies we detected microsporidial infections (Microsporidium sp.) of Daphnia and Cyclops species. We did not identified this parasites, but used the collected spores for per oral infection of laboratory culture larvae of Anopheles atroparvus. Spores were used in different ways (fresh-extracted and after storage from 1 month to 4 years in refrigerator or at room temperature (from 0 to 25 ºC). Experiments were conducted with spring, summer, autumn and winter generations of crustaceans in laboratory conditions (room temperature from 14 to 28 ºC) for periods from 14 days to till larvae perishes. In all instances infections were not detected. As a result, we did not registered peroral transmission of microsporidians of octosporic genera from blood-sucking mosquitoes to the same or another species of mosquitoes, lower crustaceans and insects.