Josh completed a Masters in Seed Technology and Business while working full time at DuPont Pioneer. His project was a literature review covering cases of field-evolved resistance to Bt crops (corn and cotton).
Bt resistance management
Dr. Doudna studied the distribution of Bt-resistant western corn rootworm (WCR) (Diabrotica virgifera virgifera) throughout Iowa, and how the evolution and spread of resistance are impacted by field management and landscape characteristics. He sampled sites throughout Iowa and used GIS to evaluate landscape and management parameters associated with the presence of resistance.
My current research projects are focused on characterization of field-evolved Bt resistance in western corn rootworm (WCR) (Diabrotica virgifera virgifera). I am involved in monitoring and quantifying evolution of Bt-resistance and cross-resistance (for Cry3Bb1, mCry3A, and Cry34/35Ab) in WCR field populations from various locations in Iowa. We use single plant bioassays, seedling mat bioassays, diet bioassays and field experiments to measure Bt resistance.