Next Generation sequencing technologies provide an outstanding resource for insect virus discovery. Genomes, transcriptomes and small RNA libraries provide evidence for the presence of viruses in a given organism. Virus transcripts in combination with small RNA data and more classical virus discovery techniques can result in identification of both overt and covert viruses, of known and unknown classification. By using this technology, we have identified several new viruses in laboratory and field colonies of various insects, and also in insect cell lines. Such viruses may provide potential new tools for suppression of pestiferous insect populations.
With funding from Monsanto Company, we are working on discovery of viruses that infect the corn rootworm toward application as biological control agents and for development of corn rootworm virus-based research tools.
Liu, S., Vijayendran, D. and Bonning, B.C. 2011. Next Generation Sequencing Technologies for Insect Virus Discovery. In “Insect Viruses”, special issue of online journal Viruses 3 (10): 1849-1869 doi:10.3390/v3101849
Vijayendran, D., Airs, P.M., Dolezal, K., Bonning, B.C. 2013. Arthropod viruses and small RNA. J. Invertebr. Pathol. 114: 186-95
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