Dicistroviruses

Virus-based strategies for management of insect pests

Several projects are underway to investigate the potential use of small RNA viruses for insect pest management.

In collaboration with Dr. W. Allen Miller, we examined the potential for use of the aphid virus, Rhopalosiphum padi virus for management of pestiferous aphids

In collaboration with Dr. Bryce Falk at UC Davis, Bonning received funding from the California Department of Food and Agriculture to work on “Development and use of recombinant Homalodisca coagulata Virus-1 for controlling Homalodisca vitripennis, the glassy-winged sharpshooter”. The glassy-winged sharpshooter is a major pest particularly of grape vines and transmits the xylem-limited bacterium, Xylella fastidiosa, the causative agent of Pierce's disease.

Several new dicistroviruses and other small RNA viruses have been identified as a result of our virus discovery work.

 

Related papers:

Boyapalle, S., Pal, N., Miller, W.A., Bonning, B.C. 2007. A glassy-winged shartpshooter cell line supports replication of Rhopalosiphum padi virus (Dicistroviridae). J. Invertebr. Pathol. 94(2): 130-139.

Pal, N., Boyapalle, S., Beckett, R., Miller, W.A. and Bonning, B.C. 2007. A baculovirus-expressed dicistrovirus that is infectious to aphids. J Virol. 81(17):9339-45.

Boyapalle, S., Beckett, R.J., Pal, N., Bonning, B.C. and Miller, W.A. 2008. Infectious genomic RNA of Rhopalosiphum padi virus transcribed in vitro from a full-length cDNA clone. Virology 375(2): 401-411.

Liu, S., Vijayendran, D. and Bonning, B.C. 2011. Next Generation Sequencing Technologies for Insect Virus Discovery. In “Insect Viruses”, special issue of online journal Viruses 3 (10): 1849-1869 doi:10.3390/v3101849

See Digital Repository @ Iowa State University to download related publications.