People

Principal Investigator

Professor
Dr. Bryony C. Bonning is a professor of entomology at Iowa State University where she is Director of the Center for Arthropod Management Technologies (CAMTech), a National Science Foundation Industry / University Cooperative Research Center that was founded in 2013.... more
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Research Associate

Assistant Scientist III
I am currently working on insect virus discovery using Next Generation Sequencing data for identification of viral sequences, from transcriptome and from small RNA sequencing datasets. I have extensive experience with bioinformatics and with optimization of bioinformatics tools for virus discovery... more
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Postdoctoral Scholar

Postdoctoral Research Associate
My work focuses on the study of insect viruses from the families Dicistroviridae and Iflaviridae I participate in a collaborative project (PIs: B. Bonning, A. Toth and W. A. Miller) directed to understand the relationship between viral infection and nutritional stress in honey ... more
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Postdoc Research Associate
My research focuses on discovery of viruses in Hemiptera using next generation sequencing methods. I am also interested in modifying the discovered viruses, which could potentially be used for management of hemipteran pests.
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My work focuses on studying the interaction of nutritional stress and viral infection in honey bees, with the goal of better understanding the roles these factors play in honey bee declines.  I am also broadly interested in insect behavior, physiology, and endocrinology, and have a strong... more
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Postdoctoral Research Associate
My current project deals with identification and optimization of toxins from Bacillus thuringiensis that have toxicity against the Asian citrus psyllid, which has had a devastating impact on the citrus industry as a vector of Huanglongbing or greening disease.
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Postdoctoral Research Associate
My current research work focuses on two major goals: 1. Identification of gut receptors in aphids that are involved in uptake of luteoviruses, and 2. Examination of mechanisms of transcytosis across the insect gut. Both of these areas of research have the potential to provide novel... more

Ph. D. Candidate

Graduate Assistant-Research
Insect viruses have developed ways to surpass the natural barrier of the gut epithelium to establish an infection in their host. This ability of viruses could be beneficially employed for insect pest management purposes, and used as a delivery system to transport important toxins to the hemocoel.... more
Mariah Kemmerer
Graduate Assistant-Research
My research interests are small non-coding RNA and the biological phenomenon of RNA interference. The specific goals of my current research are, (i) identify and characterize aphid viruses and (ii) characterize aphid micro RNAs in multiple aphid species. We use a combination of... more

Masters Candidate

Graduate Assistant-Research
My work involves modification of the Bt toxin Cry4Aa-S1 to increase toxicity against the soybean aphid for potential use in genetically modified soybean.  I am working to increase the binding of Cry4Aa-S1 to the soybean aphid gut by incorporating soybean aphid gut... more
Benjamin Deist
Graduate Assistant-Research
I am currently working on modifying Bt toxins for increased aphicidal activity, which can potentially be used for production of aphid resistant plants. The goals of my research are to (i) insert cleavage sites specific for proteases found in the aphid gut, (ii) determine if... more

Alumni

Anthony Boughton worked in the Bonning lab for his Ph.D. in Entomology from 1995 to 2001, and then continued for a year of postdoctoral research. Anthony worked on the molecular and biological characterization of a black cutworm virus, Agrotis ipsilon multiple nucleopolyhedrovirus. This virus has... more
Photogaph of Anthony Boughton
Sandhya joined the Bonning lab in 2001 and conducted research toward her PhD in Microbiology (2005). She worked on the dicistrovirus, Rhopalosiphum padi virus, and screened multiple cell lines before establishing that a glassy-winged sharpshooter cell line supports replication of this virus. She... more
Abbey Brockhouse worked as a Research Scientist with Bonning from 2001 to 2003. She worked on the ultrastructure of pericardial cells in the tobacco hornworm, Manduca sexta L. Abbey is now a 7th grade teacher in Omaha, NE.
Chun Cao worked in the Bonning lab from 2001 to 2003 as a Postdoctoral Research Associate working on the function of a putative juvenile hormone esterase binding protein, DmP29. She left the lab in 2003 to work within the industrial sector.
Nana Chougule worked in the lab from 2009 to 2012 as a postdoctoral research associate, and from 2012 to January 2014 as an Assistant Scientist III. Nana conducted research on modification of the Bacillus thuringiensis-derived cytolytic toxin, Cyt2Aa with a pea aphid gut binding peptide, to... more
Liljana worked in the Bonning lab from 2008 to 2010 as a Postdoctoral Research Associate. She worked on the aphid dicistrovirus, Rhopalosiphum padi virus, and on use of plant virus coat proteins for delivery of insect toxins in to the aphid hemocoel. She received her PhD from Wageningin UR.
Bob Harrison worked in the Bonning lab as an Assistant Scientist from 2001 to 2003, and as a postdoctoral researcher prior to that. He worked on various aspects of baculovirus biology including the genetic enhancement of baculovirus insecticides. He left the lab for a research lead position at the... more
Photograph of Robert L. Harrison
Hailing conducted her Masters research in Genetics in the Bonning lab from 1999 to 2002. She worked on the polyhedral envelope protein of Rachiplusi ou multuple nucleopolyhedrovirus. She left the Bonning lab to direct the Microarray Facility at Iowa State University.
Jeremy Kroemer worked as a Postdoctoral Research Associate for 2 years in the Bonning lab before leaving for a position at Monsanto Company in June 2013. Jeremy worked in collaboration with Dr. Bryce Falk at UC Davis on testing an infectious clone of a dicistrovirus, HoCV-1 that has potential for... more
Photograph of Jeremy Kroemer holding a lion cub
Huarong Li worked in the Bonning lab as a Postdoctoral Research Associate from 2004 to 2008. He worked on the physiological basis for the lack of toxicity of Bt toxins to aphids, and on genetic optimization of Bt toxins for enhanced binding and toxicity against aphids. Huarong left ISU in 2008 to... more
Photograph of Huarong Li
Luke Linz graduated with a degree in Microbiology in May 2013 and left the Bonning lab in August 2013 to work at Douglas Scientific in Alexandia, MN. Luke's dissertation was on identification of the first plant virus receptor in the gut of the pea aphid vector, and on plant virus - aphid molecular... more
Photograph of Lucas B. Linz
Zhiyan conducted her PhD research (Genetics) with Bonning from 2001 to 2007. She worked on characterization of the protein DmP29 in Drosophila melanogaster. The protein was initially identified as a binding protein of juvenile hormone esterase, although the significance of this interaction remains... more
Tyas worked in the Bonning lab from 2002 to 2005 for her Masters degree in Entomology. She worked on the impact of a protease expressing recombinant baculovirus on the parasitoid, Cotesia marginiventris. She went on to get her Ph.D. from University of Kentucky under the direction of Dr. Bruce Webb... more
Narinder Pal worked in the Bonning lab from 2009 to 2012 as an Assistant Scientist. She worked on demonstration that a luteovirus coat protein can be used as a delivery agent for toxins that act with the aphid hemocoel. Narinder left in 2012 to join the lab of Dr. Julie Kuhlman.
Photograph of Narinder Pal
Vijaya Paramasivan worked from 2012 to 2013 as an Assistant Scientist in the Bonning, before joining the lab of Dr. Thomas Baum in the Department of Plant Pathology and Microbiology, Iowa State University. Vijaya worked on a lepidopteran virus research project.
Photograph of Vijaya Paramasivan
Zach worked toward his Masters degree (2010) in Molecular, Cellular and Developmental Biology under the direction of Drs. W. Allen Miller and Bryony Bonning. His thesis is entitled "Translation and replication of Rhopalosiphum padi virus RNA in a plant cellular environment".
Photograph of Zachary Regelin
Nina worked in the Bonning lab from 2006 to 2009 for her Masters degree in Microbiology. She worked on the physiological basis for increased infection of black cutworms with a baculovirus (Agrotis ipsilon multiple nucleopolyhedrovirus) following ingestion of a Bt toxin (Cry1Fa2). She left the lab... more
Photograph of Nina Richtman
David worked in the Bonning lab from 2004 to 2005 on the removal of transposon target sites from the baculovirus Autographa californca multiple nucleopolyhedrovirus fg25k gene. Transposon insertion into these target sites results in accumulation of a few polyhedra (fp) phenotype. It was later shown... more
Photograph of David Sandgren
Shan worked in the Bonning lab as a Postdoctoral Research Associate from 1997 to 1999. He worked on screening of a pericardial cell phage display library for proteins that bound juvenile hormone esterase. This work provided the basis for analysis of the function of the protein DmP29 in the model... more
Siva worked in the Bonning lab from 2005 to 2007 as a Postdoctoral Research Associate. He worked on baculovirus expression of the Pea enation mosaic virus coat protein for production of virus-like particles in insect cell lines, and on use of the coat protein to delivery GFP from the aphid gut into... more
Photograph of S. Sivakumar
Wendy Sparks worked toward her PhD in Genetics in the Bonning lab from 2005 to 2010. She worked on interaction of baculovirus occlusion-derived virus envelope proteins (ODV-E56 and ODV-E66) with the midgut microvilli of the tobacco budworm, Heliothis virescens. Wendy left the lab for a postdoctoral... more
Photograph of Wendy Sparks
Hailin worked toward his Masters thesis in Toxicology from 2004 to 2008 in the Bonning lab. He worked on the tissue specificity of a baculovirus-expressed, basement membrane-degrading protease in larvae of the tobacco budworm, Heliothis virescens.   Hailin's MS thesis: Tissue specificity... more
Photograph of Hailin Tang