Insecticide Toxicology: ENT 675

Instructor: Joel Coats	3 Credits
Lectures Monday and Wednesday 10:00 a.m.	433 Science II
Laboratory Friday 10:00 a.m.-1:00 p.m.	43 Science II; 114 Insectary

• Text: Toxicology of Insecticides by Fumio Matsumara, Plenum Press

Lecture Topics

• Introduction
• Toxicological concepts
• History of insecticides
• Chlorinated hydrocarbons
• Chlorinated hydrocarbons
• Organophosphates
• Organophosphates
• Organophosphates
• Carbamates
• Carbamates

• EXAM I

• Pyrethroids
• Pyrethroids
• Formamidines
• Insect growth regulators
• Microbial insecticides
• Botanicals/natural products
• Metabolic inhibitors

• EXAM II

• Metabolism of insecticides
• Resistance to insecticides
• Genetics of resistance (Dr. Krafsur)
• Environmental effects
• Environmental fate
• Human health aspects
• Acaricides, molluscicides, piscicides, and rodenticides
• Biotechnology discussion

• EXAM III
• LAB EXAM

Laboratory Topics

• Introduction to the Laboratory
• Synthesis of ethoxychlor
• Purification/thin-layer chromatography
• Crystallization and Characterization (m.p., NMR, mass spec)
• Bioassay: solutions, techniques, range-finding
• Bioassay: LD50 and LC50 dosing
• Bioassay calculation
• Residue analysis: overview and extraction
• Residue analysis: clean up and concentration of extract
• Residue analysis: chromatography theory/std curve prep.
• Residue analysis: gas chromatography/HPLC
• Metabolism and mobility of pesticides in soil
• Radiotracer theory and methodology
• Radiotracer applications

Grading