Insect Pathology: ENT 673

Instructor: Bryony Bonning	Spring Semester 2001
4 Credits	433 Science II
Lectures Monday, Wednesday, Friday 8:00-8:50	Lab Thursday 12:00-3:00 pm

• Text: Tanada and Kaya 1993 Insect Pathology
• This course is offered alternate Spring semesters.

Course Schedule

Week 1	Scope of the course:
	History of insect pathology
	Characteristics of insect pathogens
	Types of insect diseases: Definitions
	Diagnosis of insect diseases
Week 2	Non pathogenic microorganisms
	Genetic engineering of symbiotic bacteria
	LAB 1: Use of the microscope
	Bacterial fauna of healthy insects
Week 3	Protozoa: General characteristics
	Non-spore formers: Flagellates, Ciliates and Amoeba
	Spore formers: Gregarines, Coccidia, Microsporidia
	Microsporidia
Week 4	Applied Aspects of Microsporidia
	Protozoa: Slides/Discussion
	Techniques in Insect Pathology
	LAB 2: Protozoa
Week 5	Bacteria: General characteristics
	Non-spore forming Bacteria
	Historical Aspects of Bacillus
	LAB 3:Bacteria I
Week 6	Spore-forming Bacteria
	Bacillus thuringiensis
	LAB 4: Bacteria II
Week 7	Biotechnology and Bt
	Resistance to Bt
	Nematodes: General characteristics and biology of nematode groups
Week 8	EXAM I: Protozoa, Bacteria
	Nematodes: Steinernematidae, Heterorhabditae
	Nematodes: Insects as vectors for nematode parasites of vertebrates
	Genetic engineering of nematodes
	LAB 5: Nematodes
SPRING BREAK
Week 9	Fungi: General characteristics:
	Mastigomycetes
	Applied aspects of Beauveria bassiana
	LAB 6: Fungi
Week 10	Fungi: Zygomycetes, Ascomycetes
	Basidiomycetes, Deuteromyces
Week 11	Viruses: Introduction
	Baculoviruses (NPV, GV)
	Other insect viruses (denso-, arboviruses)
	LAB 7: Viruses I: Characteristics of NPV and GV
Week 12	EXAM II: Nematodes and Fungi
	Other ds DNA viruses
	RNA viruses
	LAB 8: Viruses II: Virus infection and dissemination
Week 13	Molecular Biology of Baculoviruses
	Recombinant Baculovirus Insecticides
	Electron microscopy
	LAB 9: Preparation of material for EM and TEM demonstration
Week 14	Insect Defenses against Pathogens
	Epizootiology
	Microbial Control
Week 15	Student presentations
Week 16	FINAL EXAM

Course Grading

• Essay (15%)
• Grant proposal (15%)
• Presentation (10%)
• Lab writeups (10%)
• Exam I (15%)
• Exam II (15%)
• Final Comprehensive Exam (20%)